Year: 2015 | Month: March | Volume 8 | Issue 1
Genetic diversity studies in rice (Oryza Sativa L.) using microsatellite markers
DOI:10.5958/2230-732X.2015.00019.4
Abstract:
The objective of present study was to evaluate the genetic polymorphism and identification of diverse parents among the 76 rice accessions using simple sequences repeat (SSR) markers. The accessions showed significant phenotypic variation for all the characters analyzed. The SSR Markers were highly polymorphic across all accessions and altogether 79 alleles were detected. The overall Polymorphic information content (PIC) value ranged from 0.26 to 0.65 with an average of 2.82 per locus indicting high level of genetic variation. The cluster analysis showed the rice germplasm accessions grouped in to two major groups and 14 subgroups. The pair-wise genetic dissimilarity co-efficient indicated that the highest dissimilarity was obtained between cultivar B.3688-TB and IR.67017-1(0.1935) followed by cultivar Badi Kodi and Changhat (0.3333). These grmplasms were showing wide genetic divergence among the constituent in it and may be directly utilized in hybridization programme for improvement of yield related traits. The markers RM 413, RM 481, RM 206 and RM 20 produced a maximum of four alleles. These microsatellite markers could serve as a powerful tool in selecting genetically diverse germplasm accessions, to execute efficient selection in highly segregating generations.
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